P-104: Embryo Sex Selection by Migration of Spermatozoa in Different Acidic pH Environment

Background: Today factors including cultural, social, religious, economic and medical such as X Linked diseases encouraged couples to choose traditional methods or new treatment for selecting the gender of child. It seems that Spermatozoa containing chromosomes X and Y have different speed in various pH. In this study the velocity of spermatozoa in different acidic pH was evaluated. Materials and Methods: A number of 60 mice (NMRI) were divided in a control group and three experimental groups. Female mice stimulated by injecting PMSG matter to super ovulation. Oocyte retrieved under sterile condition from oviduct. Spermatozoa provided from cauda epididymis and deferane and entered on media by pH 6.7، 6.8 and 6.9. The fastest spermatozoa were use for IVF. Produced embryo were transplanted to pseudo pregnant mother. The female and male infants were counted after birth. Analysis of data done with the help of SPSS, Anova Software. Results: The proportion of female to male in control group; experimental group1(pH=6.7); experimental group 2 (pH=6.8); and experimental group 3 (pH=6.9) were (Mean ± SD) 49.54 ± 2.83, 66.20 ± 3.37, 65.76 ± 3.76, 63.31 ± 4.12 respectively. The comparison of mean of proportion of female to male infant in control group and experimental groups showed significant difference(p≤0.05). There was not significant difference between mean of proportion of female to male infant in experimental groups(p≥0.05). The Duncan and Tuky test were the same. Conclusion: The results showed that the speed of spermatozoa X in acidic media is higher and this technique is cheap and accessible.